Linear TT1 (Lin TT1) peptide

Linear TT1 (Lin TT1) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-AKRGARSTA-OH

H-Ala-Lys-Arg-Gly-Ala-Arg-Ser-Thr-Ala-OH

9

95.81%

C₃₆H₆₈N₁₆O₁₂

917.02

Synthetic

Powder

The Linear TT1 (Lin TT1) peptide (sequence: AKRGARSTA) has high efficiency in the penetration and placement of the nanosystem in the tumor.

Linear TT1 peptide (sequence: AKRGARSTA) is a remarkable innovation in the field of cancer treatment. It is designed to be a key component of an advanced tumor-penetrating nanosystem. This peptide exhibits outstanding tumor homing and penetration capabilities. In MCF10CA1a breast tumors, it enables nanoparticles (NPs) to accumulate effectively in extravascular tumor tissue, with much of the signal detected outside the tumor blood vessels. Notably, it shows superior performance compared to other peptides like CGKRK. The peptide binds to p32 protein with a Kd of 8.7 μM, and its behavior as a cryptic CendR peptide allows for a unique mechanism of action. When conjugated to NPs, it confers the ability to target not only tumor vessels but also tumor-associated macrophages and cells, making it a highly promising candidate for enhanced tumor treatment strategies.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sharma S, Kotamraju VR, Mölder T, Tobi A, Teesalu T, Ruoslahti E. Tumor-Penetrating Nanosystem Strongly Suppresses Breast Tumor Growth. Nano Lett. 2017 Mar 8;17(3):1356-1364. doi: 10.1021/acs.nanolett.6b03815. Epub 2017 Feb 17. PMID: 28178415; PMCID: PMC5819594.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.