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Product Name
Lin28B-specific antibody
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Description
Lin28B-specific Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human placenta tissue, human spleen tissue. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CSDD2 antibody; FLJ16517 antibody; lin 28 homolog B (C. elegans) antibody; Lin 28B antibody; Lin28 antibody; LIN28B antibody; Protein lin 28 homolog B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001004317). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human testis using Catalog No:112234(Lin28B-specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112234(Lin28B-specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112234(Lin28B-specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112234(Lin28B-specific Antibody) at dilution of 1:1000
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Background
LIN28B, also named as CSDD2, belongs to the lin-28 family. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Lin2B is stage-specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells; however, it is suppressed in most adult tissues except for skeletal and cardiac muscle. Lin28B shares 78% sequence identity with LIN28A, with some unknown functions. This antibody is specific to LIN28B.
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References
- Gaytan F, Sangiao-Alvarellos S, Manfredi-Lozano M. Distinct expression patterns predict differential roles of the miRNA-binding proteins, Lin28 and Lin28b, in the mouse testis: studies during postnatal development and in a model of hypogonadotropic hypogonadism. Endocrinology. 154(3):1321-36. 2013.
- Yoshikawa T, Otsuka M, Kishikawa T. Unique haploinsufficient role of the microRNA-processing molecule Dicer1 in a murine colitis-associated tumorigenesis model. PloS one. 8(9):e71969. 2013.
- Wu T, Jia J, Xiong X. Increased expression of Lin28B associates with poor prognosis in patients with oral squamous cell carcinoma. PloS one. 8(12):e83869. 2013.
- Sangiao-Alvarellos S, Manfredi-Lozano M, Ruiz-Pino F. Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty. Scientific reports. 5:15683. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"