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Product Name
LIMS2 Polyclonal Antibody
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Description
Polyclonal antibody to LIMS2
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Tested applications
WB
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Species reactivity
Human, Rat
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Alternative names
LIMS2 antibody; LGMD2W antibody; PINCH-2 antibody; PINCH2 antibody; LIM zinc finger domain containing 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human LIMS2 (NP_001154875.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - LIMS2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LIMS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Plays a role in modulating cell spreading and migration.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"