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Product Name
LIG4 Polyclonal Antibody
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Description
Polyclonal antibody to LIG4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
LIG4 antibody; LIG4S antibody; DNA ligase 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - LIG4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LIG4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - LIG4 Polyclonal Antibody
Immunofluorescence analysis of U-2OS cells using LIG4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"