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Product Name
LGTN antibody
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Description
LGTN Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, PC-3 cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HCA56 antibody; LGTN antibody; ligatin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LGTN recombinant protein (Accession Number: NM_006893). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112213(LGTN antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using LGTN antibody Catalog No:112213 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
LGTN represents ligatin
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References
- Starck SR, Jiang V, Pavon-Eternod M. Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I. Science (New York, N.Y.). 336(6089):1719-23. 2012.
- Skabkin MA, Skabkina OV, Dhote V, Komar AA, Hellen CU, Pestova TV. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes & development. 24(16):1787-801. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"