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Product Name
LGALS3BP antibody
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Description
LGALS3BP Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human skin tissue. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
90K antibody; BTBD17B antibody; Galectin 3 binding protein antibody; LGALS3BP antibody; M2BP antibody; Mac 2 binding protein antibody; MAC 2 BP antibody; MAC2BP antibody; Tumor assOCiated antigen 90K antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LGALS3BP recombinant protein (Accession Number: NM_005567). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112204(LGALS3BP antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:112204(LGALS3BP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:112204(LGALS3BP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112204(LGALS3BP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP is a widely expressed, secreted galectin. It has been found elevated in the serum of patients with various types of breast, lung, colorectal, ovary, and endometrial cancer, and in those infected by the human immunodeficiency virus (HIV). High expression levels of LGALS3BP are associated with a shorter survival, the occurrence of metastasis or a reduced response to chemotherapy. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Immunoblotting showed that LGALS3BP is likely contained in breast milk, serum, semen, saliva, urine, and tears.
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