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Product Name
LFNG Polyclonal Antibody
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Description
Polyclonal antibody to LFNG
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
LFNG antibody; SCDO3 antibody; beta-1,3-N-acetylglucosaminyltransferase lunatic fringe antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - LFNG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LFNG antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.
Immunohistochemistry - LFNG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using LFNG antibody at dilution of 1:100 (40x lens).
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Background
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity). Decreases the binding of JAG1 to NOTCH2 but not that of DLL1. Essential mediator of somite segmentation and patterning (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"