Lf-CATH1 peptide

Lf-CATH1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

PPCRGIFCRRVGSSSAIARPGKTLSTFITV

Pro-Pro-Cys-Arg-Gly-Ile-Phe-Cys-Arg-Arg-Val-Gly-Ser-Ser-Ser-Ala-Ile-Ala-Arg-Pro-Gly-Lys-Thr-Leu-Ser-Thr-Phe-Ile-Thr-Val

30

96%

Synthetic

Powder

39.0% similar to Gallinacin 3. Active against Gram-positive S. aureus (ATCC2592, IS1303, IS 1349) (MIC 1.25-10 uM), S. epidermidis (MIC 5 uM), P. acnes ATCC11827 (MIC 10.1 uM), Gram-negative P. aeruginosa (IS 1411, IS 1412, IS 1413) (MIC 10. 1 uM), E. coli (ATCC25922, IS 1334, IS 1335, IS 1342) (MIC 2.5-5.0 uM), K. pneumoniae IS 1368 (MIC 10.1 uM), and fungi C. albicans ATCC2002, C. glabrata IS0902) (MIC 1.26-5.04 uM). Lf-CATH1 was found in spleen, liver and kidney, fragile large-headed frog, fragile wart frog, Limnonectes fragilis, Hainan, China, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Yu H, Cai S, Gao J, et al. Identification and polymorphism discovery of the cathelicidins, Lf-CATHs in ranid amphibian (Limnonectes fragilis). FEBS J. 2013;280(23):6022-32.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.