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Product Name
LETM1 antibody
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Description
LETM1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, Jurkat cells, MCF7 cells. Positive IHC detected in human ovary tumor tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 83 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LETM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LETM1 recombinant protein (Accession Number: NM_012318). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112201(LETM1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112201(LETM1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112201(LETM1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using LETM1 antibody Catalog No:112201 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-LETM1 (IP:Catalog No:112201, 4ug; Detection:Catalog No:112201 1:500) with HEK-293 cells lysate 3200ug.
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Background
LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.
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References
- Jiang D, Zhao L, Clish CB, Clapham DE. Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome. Proceedings of the National Academy of Sciences of the United States of America. 110(24):E2249-54. 2013.
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