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Product Name
LDHA-Specific antibody
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Description
LDHA-Specific Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human heart tissue, human skeletal muscle tissue, human testis tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in A431 cells, Jurkat cells, mouse testis tissue, rat kidney tissue. Observed molecular weight by Western-blot: 37 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
lactate dehydrogenase A antibody; LDH A antibody; LDH M antibody; LDH muscle subunit antibody; LDH1 antibody; LDHA antibody; PIG19 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005566). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:112183(LDHA-Specific antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human heart using Catalog No:112183(LDHA-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:112183(LDHA-Specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112183(LDHA-Specific Antibody) at dilution of 1:100 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-LDHA-Specific (IP:Catalog No:112183, 4ug; Detection:Catalog No:112183 1:2000) with mouse kidney tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (Catalog No:112183, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has 5 isoforms and can form a homotetramer(PMID:11276087) .The antibody has no cross reaction to LDHB and LDHC.This antibody is specific to LDHA.
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References
- Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
- Xian ZY, Liu JM, Chen QK. Inhibition of LDHA suppresses tumor progression in prostate cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(10):8093-100. 2015.
- He TL, Zhang YJ, Jiang H, Li XH, Zhu H, Zheng KL. The c-Myc-LDHA axis positively regulates aerobic glycolysis and promotes tumor progression in pancreatic cancer. Medical oncology (Northwood, London, England). 32(7):187. 2015.
- Zhao M, Fang W, Wang Y. Enolase-1 is a therapeutic target in endometrial carcinoma. Oncotarget. 6(17):15610-27. 2015.
- Liu X, Yao D, Liu C. Overexpression of ABCC3 promotes cell proliferation, drug resistance, and aerobic glycolysis and is associated with poor prognosis in urinary bladder cancer patients. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016.
- Dong SY, Guo YJ, Feng Y. The epigenetic regulation of HIF-1α by SIRT1 in MPP(+) treated SH-SY5Y cells. Biochemical and biophysical research communications. 470(2):453-9. 2016.
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