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Product Name
LDHA antibody
- Documents
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Description
LDHA Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human skeletal muscle tissue. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, A375 cells, HEK-293 cells, HepG2 cells, human testis tissue, MCF7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 32-36 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
lactate dehydrogenase A antibody; LDH A antibody; LDH M antibody; LDH muscle subunit antibody; LDH1 antibody; LDHA antibody; PIG19 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LDHA recombinant protein (Accession Number: NM_005566). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112181(LDHA antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:112181(LDHA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:112181(LDHA antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using LDHA antibody Catalog No:112181 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LDHA (IP:Catalog No:112181, 4ug; Detection:Catalog No:112181 1:500) with HEK-293 cells lysate 2000ug.
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Background
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has 5 isoforms and can form a homotetramer(PMID:11276087).
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References
- Wei W, Hu YY. Expression of hypoxia-regulated genes and glycometabolic genes in placenta from patients with intrahepatic cholestasis of pregnancy. Placenta. 35(9):732-6. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"