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Product Name
LCP1 Polyclonal Antibody
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Description
Polyclonal antibody to LCP1
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Tested applications
WB, IP
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Species reactivity
Human, Mouse
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Alternative names
LCP1 antibody; CP64 antibody; HEL-S-37 antibody; L-PLASTIN antibody; LC64P antibody; LPL antibody; PLS2 antibody; plastin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LCP1 (NP_002289.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - LCP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LCP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunoprecipitation - LCP1 Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug LCP1 antibody . Western blot was performed from the immunoprecipitate using LCP1 antibody at a dilition of 1:1000.
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Background
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"