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Product Name
LCE1B antibody
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Description
LCE1B Rabbit Polyclonal antibody. Positive WB detected in A375 cells, mouse skin tissue, rat skin tissue. Positive IHC detected in human skin tissue, human oesophagus tissue. Positive IF detected in A375 cells. Observed molecular weight by Western-blot: 10-12 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
late cornified envelope 1B antibody; Late envelope protein 2 antibody; LCE1B antibody; LEP2 antibody; SPRL2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LCE1B recombinant protein (Accession Number: NM_178349). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:112170(LCE1B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human skin using Catalog No:112170(LCE1B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:112170(LCE1B antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A375 cells using Catalog No:112170(LCE1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"