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LC3A/B antibody

Cat.#: 107373

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Product Information

  • Product Name
    LC3A/B antibody
  • Documents
  • Description
    LC3A/B Mouse Monoclonal antibody. Positive IHC detected in human skeletal muscle tissue, human liver tissue. Positive FC detected in HeLa cells. Positive WB detected in human brain tissue, HeLa/HepG2 cells, pig brain tissue. Observed molecular weight by Western-blot: 14 kDa, 16 kDa
  • Tested applications
    ELISA, IHC, WB, FC
  • Species reactivity
    Human, Pig; other species not tested.
  • Alternative names
    LC3 antibody; LC3A antibody; LC3A/B antibody; LC3B antibody; MAP1A/MAP1B LC3 A antibody; MAP1A/MAP1B light chain 3 A antibody; MAP1ALC3 antibody; MAP1BLC3 antibody; MAP1LC3A antibody; MAP1LC3A/B antibody; MAP1LC3B antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of recombinant protein (Accession Number: NM_032514). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    Western Blot analysis of MAP1LC3A/B in HeLa and HepG2 cells untreated/treated with 50μM chloroquine overnight using Catalog No:107373 (MAP1LC3A/B monoclonal antibody) at dilution of 1:1000.

    Western Blot analysis of MAP1LC3A/B in HeLa and HepG2 cells untreated/treated with 50μM chloroquine overnight using Catalog No:107373 (MAP1LC3A/B monoclonal antibody) at dilution of 1:1000.

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107373(LC3A/B antibody) at dilution of 1:1000

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107373(LC3A/B antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:107373(LC3A/B Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:107373(LC3A/B Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:107373(LC3A/B Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:107373(LC3A/B Antibody) at dilution of 1:50

    1X10^6 HeLa cells were stained with 0.2ug LC3A/B antibody (Catalog No:107373, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug LC3A/B antibody (Catalog No:107373, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
  • References
    • Yang XL, Lin FJ, Guo YJ, Shao ZM, Ou ZL. Gemcitabine resistance in breast cancer cells regulated by PI3K/AKT-mediated cellular proliferation exerts negative feedback via the MEK/MAPK and mTOR pathways. OncoTargets and therapy. 7:1033-42. 2014.
    • Yuan X, Du J, Hua S. Suppression of autophagy augments the radiosensitizing effects of STAT3 inhibition on human glioma cells. Experimental cell research. 330(2):267-76. 2015.
    • Wang LQ, Cheng XS, Huang CH, Huang B, Liang Q. Rapamycin protects cardiomyocytes against anoxia/reoxygenation injury by inducing autophagy through the PI3k/Akt pathway. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 35(1):10-5. 2015.
    • Wu P, Zhu X, Jin W, Hao S, Liu Q, Zhang L. Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells. Biochemical and biophysical research communications. 460(2):183-90. 2015.
    • Li Z, Wang Y, Newton IP, Zhang L, Ji P, Li Z. GRP78 is implicated in the modulation of tumor aerobic glycolysis by promoting autophagic degradation of IKKβ. Cellular signalling. 27(6):1237-45. 2015.
    • Sun Y, Liu Z, Zou X. Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer. Journal of bioenergetics and biomembranes. 47(4):319-29. 2015.
    • Dai C, Tang S, Velkov T, Xiao X. Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy. Molecular neurobiology. 2015.
    • Gao L, Wang Y, Xu Z. SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells. Apoptosis : an international journal on programmed cell death. 20(12):1636-50. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"