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Product Name
LAP3 antibody
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Description
LAP3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, mouse heart tissue, SH-SY5Y cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 56kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cytosol aminopeptidase antibody; LAP antibody; LAP 3 antibody; LAP3 antibody; LAPEP antibody; leucine aminopeptidase 3 antibody; Leucyl aminopeptidase antibody; PEPS antibody; Peptidase S antibody; Proline aminopeptidase antibody; Prolyl aminopeptidase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LAP3 recombinant protein (Accession Number: NM_015907). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112142(LAP3 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112142(LAP3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112142(LAP3 antibody) at dilution of 1:50 (under 40x lens)
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Background
LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684).
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References
- Zhu Z, Xu W, Dai J. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. The international journal of biochemistry & cell biology. 45(3):571-82. 2013.
- Huang S, Cui Y, Guo X. 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats. Reproductive toxicology (Elmsford, N.Y.). 51:114-24. 2015.
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