-
Product Name
Lamin B1 antibody
- Documents
-
Description
Lamin B1 Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive FC detected in HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 66 kDa
-
Tested applications
ELISA, WB, IF, FC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ADLD antibody; lamin B1 antibody; LMN antibody; LMN2 antibody; LMNB antibody; LMNB1 antibody
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of Lamin B1 recombinant protein (Accession Number: NM_005573). Purification method: Protein G purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: 1:5000-1:50000
IF: 1:20-1:200
-
Validations
Western blot on multiple cells/tissues with anti-LMNB1 (Catalog No:117328) at dilution 1:20000.
Immunofluorescent analysis of HepG2 cells using Catalog No:117328(Lamin B1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
1X10^6 HeLa cells were stained with 0.2ug Lamin B1 antibody (Catalog No:117328, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.
Western blot of Hela cell with anti-LMNB1 (Catalog No:117328) at various dilutions.
IP Result of anti-Lamin B1 (IP:Catalog No:117328, 4ug; Detection:Catalog No:117328 1:20000) with HeLa cells lysate 3560ug.
-
Background
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
-
References
- Tan T, Lu B, Zhang J. Notch1 signaling antagonizes transforming growth factor-β pathway and induces apoptosis in rabbit trophoblast stem cells. Stem cells and development. 23(8):813-22. 2014.
- Chen L, Zhang W, Liang HF. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells. Cell communication and signaling : CCS. 12:18. 2014.
- Song E, Yu W, Xiong X, Kuang X, Ai Y, Xiong X. Astrocyte elevated gene-1 promotes progression of cervical squamous cell carcinoma by inducing epithelial-mesenchymal transition via Wnt signaling. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society. 25(3):345-55. 2015.
- Liu H, Wang ZC, Bai YG. Simulated microgravity promotes monocyte adhesion to rat aortic endothelium via nuclear factor-κB activation. Clinical and experimental pharmacology & physiology. 42(5):510-9. 2015.
- Bai L, Zhang W, Tan L. Hepatitis B virus hijacks CTHRC1 to evade host immunity and maintain replication. Journal of molecular cell biology. 7(6):543-56. 2015.
- Chen H, Sun Y, Lai L. Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice. Neuroscience. 308:157-68. 2015.
- Zhang J, Li T, Ji W, Yu Y, Tan T. Rho GDIalpha Modulates Rabbit Trophoblast Stem Cell Survival and Migration. Biology of reproduction. 93(6):144. 2015.
- Nie D, Zhang D, Dai J. Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction. 94(2):31. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"