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Product Name
Lactoferrin antibody
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Description
Lactoferrin Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human pancreas tissue. Positive IP detected in HeLa cells. Positive IHC detected in human liver tissue, human prostate hyperplasia tissue. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
GIG12 antibody; HLF2 antibody; Lactoferrin antibody; lactotransferrin antibody; LTF antibody; Talalactoferrin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Lactoferrin recombinant protein (Accession Number: NM_002343). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112266(LTF antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:112266(LTF antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112266(LTF antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-LTF (IP:Catalog No:112266, 4ug; Detection:Catalog No:112266 1:600) with HeLa cells lysate 4000ug.
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Background
LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an Mr of 30 kDa and a C-terminal tryptic fragment having an Mr of 50 kDa by a mild tryptic digest(PMID:3790094).
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