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Product Name
L3MBTL3 Polyclonal Antibody
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Description
Polyclonal antibody to L3MBTL3
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse
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Alternative names
L3MBTL3 antibody; MBT-1 antibody; MBT1 antibody; lmbt-like 3 (Drosophila) antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - L3MBTL3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.
Immunofluorescence - L3MBTL3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - L3MBTL3 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody . Western blot was performed from the immunoprecipitate using L3MBTL3 antibody at a dilition of 1:1000.
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Background
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"