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Product Name
L3MBTL1 Polyclonal Antibody
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Description
Polyclonal antibody to L3MBTL1
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Tested applications
IF
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Species reactivity
Human
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Alternative names
L3MBTL1 antibody; H-LMBT antibody; L3MBTL antibody; ZC2HC3 antibody; dJ138B7.3 antibody; lmbt-like 1 (Drosophila) antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human L3MBTL1 (NP_056293.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
IF 1:50 - 1:100
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Validations
Immunofluorescence - L3MBTL1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using L3MBTL1 antibody .
Immunofluorescence - L3MBTL1 Polyclonal Antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using L3MBTL1 antibody .Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue:DAPI for nuclear staining.RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Background
Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
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