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Product Name
Kynurenine 3 monooxygenase antibody
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Description
Kynurenine 3 monooxygenase Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, human heart tissue, mouse brain tissue, SKOV-3 cells. Positive IHC detected in human heart tissue, human liver tissue, human ovary tissue, human skin tissue. Positive IF detected in C6 cells. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
dJ317G22.1 antibody; KMO antibody; Kynurenine 3 hydroxylase antibody; Kynurenine 3 monooxygenase antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Kynurenine 3 monooxygenase recombinant protein (Accession Number: BC005297). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human heart using Catalog No:107367(KMO antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107367(KMO antibody) at dilution of 1:50 (under 40x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107367(KMO Antibody) at dilution of 1:1000
Immunofluorescent analysis of C6 cells using Catalog No:107367(KMO Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
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Background
KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).
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References
- Bosnyák E, Kamson DO, Guastella AR. Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas. Neuro-oncology. 17(9):1284-92. 2015.
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