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Product Name
KYNU antibody
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Description
KYNU Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 52kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KYNU antibody; Kynureninase antibody; L kynurenine hydrolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KYNU recombinant protein (Accession Number: XM_047446252). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112260(KYNU antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112260(KYNU antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using KYNU antibody Catalog No:112260 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD kD homodimer predominantly located in the cytoplasm(PMID:9180257). It has 2 isoforms produced by alternative splicing.
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