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Product Name
KU70 antibody
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Description
KU70 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, rat testis tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, A549 cells, MCF7 cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
5 dRP lyase Ku70 antibody; 70 kDa subunit of Ku antigen antibody; CTC75 antibody; CTCBF antibody; DNA repair protein XRCC6 antibody; G22P1 antibody; KU70 antibody; ML8 antibody; Thyroid lupus autoantigen antibody; TLAA antibody; XRCC6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KU70 recombinant protein (Accession Number: NM_001469). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112254(KU70,XRCC6 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded rat testis using Catalog No:112254(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using XRCC6 antibody Catalog No:112254 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug KU70,XRCC6 antibody (Catalog No:112254, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-KU70,XRCC6 (IP:Catalog No:112254, 3ug; Detection:Catalog No:112254 1:1000) with HeLa cells lysate 1600ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112254(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)
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Background
X-ray repair cross-complementing protein 6（XRCC6）gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
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References
- Zhou H, Mu X, Chen J. RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549. International journal of radiation biology. 89(9):708-15. 2013.
- Dong H, Shi Q, Song X. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Toxicology and applied pharmacology. 286(1):10-6. 2015.
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