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Product Name
KRT81 Polyclonal Antibody
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Description
Polyclonal antibody to KRT81
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
KRT81 antibody; HB1 antibody; Hb-1 antibody; KRTHB1 antibody; MLN137 antibody; ghHkb1 antibody; hHAKB2-1 antibody; keratin 81 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-390 of human KRT81 (NP_002272.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - KRT81 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KRT81 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - KRT81 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using KRT81 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - KRT81 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using KRT81 antibody at dilution of 1:100 (40x lens).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"