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Product Name
KRT81 antibody
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Description
KRT81 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, human spleen tissue, mouse thymus tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ghHb1 antibody; ghHkb1 antibody; Hair keratin K2.9 antibody; Hb 1 antibody; HB1 antibody; hHAKB2 1 antibody; K81 antibody; keratin 81 antibody; Keratin antibody; hair antibody; basic antibody; Keratin antibody; type II cuticular Hb1 antibody; KRT81 antibody; KRTHB1 antibody; MLN 137 antibody; MLN137 antibody; Type II hair keratin Hb1 antibody; Type II keratin Kb21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KRT81 recombinant protein (Accession Number: BC021241). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112136(KRT81 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112136(KRT81 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using KRT81 antibody Catalog No:112136 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT81 is a type II keratin.
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