Product Name

KRT33B antibody

Documents

Description

KRT33B Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, human brain tissue. Positive IHC detected in human prostate cancer. Observed molecular weight by Western-blot: 46 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

human; other species not tested.

Alternative names

KRT33B antibody; hHa3 II antibody; KRTHA3A antibody; keratin 33B antibody; Ha 3II antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of KRT33B recombinant protein (Accession Number: NM_002279). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

Validations

MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:112130(KRT33B antibody) at dilution of 1:800

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112130(KRT33B antibody) at dilution of 1:50 (under 10x lens)

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

References

Ma YM, Wu KJ, Dang Q. Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling. Asian journal of andrology. 16(6):817-23. 2014.

KRT33B antibody

Product Information

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