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Product Name
KRT23 antibody
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Description
KRT23 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, HeLa cells, HepG2 cells. Positive IHC detected in human colon cancer tissue, human skin cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CK 23 antibody; CK23 antibody; Cytokeratin 23 antibody; DKFZp434G032 antibody; HAIK1 antibody; K23 antibody; Keratin 23 antibody; KRT23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KRT23 recombinant protein (Accession Number: XM_047435728). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112129(KRT23 antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:112129(KRT23 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:112129(KRT23 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:112129(KRT23 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT 23 belongs to type I keratins.
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