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Product Name
KPNA2 Polyclonal Antibody
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Description
Polyclonal antibody to KPNA2
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse
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Alternative names
KPNA2 antibody; IPOA1 antibody; QIP2 antibody; RCH1 antibody; SRP1-alpha antibody; SRP1alpha antibody; importin subunit alpha-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - KPNA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KPNA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - KPNA2 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using KPNA2 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - KPNA2 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody . Western blot was performed from the immunoprecipitate using KPNA2 antibody at a dilition of 1:1000.
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Background
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
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