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Product Name
KLK10 Polyclonal Antibody
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Description
Polyclonal antibody to KLK10
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
KLK10 antibody; NES1 antibody; PRSSL1 antibody; kallikrein-10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-276 of human KLK10 (NP_001070968.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - KLK10 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KLK10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - KLK10 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using KLK10 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - KLK10 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using KLK10 antibody . Blue: DAPI for nuclear staining.
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Background
Has a tumor-suppressor role for NES1 in breast and prostate cancer.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"