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Product Name
KLHL14-Specific antibody
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Description
KLHL14-Specific Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human cervical cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse ovary tissue, mouse testis tissue, rat brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 39-44 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
kelch like 14 (Drosophila) antibody; Kelch like protein 14 antibody; KIAA1384 antibody; KLHL14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020805). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112093(KLHL14-Specific antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using KLHL14 antibody Catalog No:112093 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:112093(KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112093(KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens)
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Background
Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"