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Product Name
KLF9 Polyclonal Antibody
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Description
Polyclonal antibody to KLF9
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
KLF9 antibody; BTEB antibody; BTEB1 antibody; Kruppel like factor 9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human KLF9 (NP_001197.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - KLF9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KLF9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - KLF9 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using KLF9 antibody . Blue: DAPI for nuclear staining.
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Background
Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Acts as an epidermal circadian transcription factor regulating keratinocyte proliferation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"