-
Product Name
KLF2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to KLF2
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
KLF2 antibody; LKLF antibody; Kruppel like factor 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KLF2 (NP_057354.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - KLF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KLF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90S.
Western blot - KLF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines,using KLF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunofluorescence - KLF2 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using KLF2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KLF2 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using KLF2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KLF2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using KLF2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"