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Product Name
KLF10 antibody
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Description
KLF10 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
EGR alpha antibody; EGRA antibody; KLF10 antibody; Krueppel like factor 10 antibody; Kruppel like factor 10 antibody; TIEG antibody; TIEG 1 antibody; TIEG1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KLF10 recombinant protein (Accession Number: NM_001032282). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112076(KLF10 antibody) at dilution of 1:100
IP Result of anti-KLF10 (IP:Catalog No:112076, 4ug; Detection:Catalog No:112076 1:300) with Jurkat cells lysate 2800ug.
Immunofluorescent analysis of HeLa cells using Catalog No:112076(KLF10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.
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