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Product Name
KLF1 Polyclonal Antibody
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Description
Polyclonal antibody to KLF1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
KLF1 antibody; EKLF antibody; CDAN4 antibody; HBFQTL6 antibody; INLU antibody; Kruppel like factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF1 (NP_006554.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - KLF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KLF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
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Background
Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
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