KKFFVLK peptide
Not For Human Use, Lab Use Only.
Cat.#: 319746
Special Price 101.7 USD
-
Product Name
KKFFVLK peptide
-
Documents
Batch to batch variation of the purity
-
Sequence Shortening
H-KKFFVLK-OH
-
Sequence
H-Lys-Lys-Phe-Phe-Val-Leu-Lys-OH
-
Length (aa)
7
-
Peptide Purity (HPLC)
95.96%
-
Molecular Formula
C47H76N10O8
-
Molecular Weight
909.17
-
Source
Synthetic
-
Form
Powder
-
Description
KKFFVLK polypeptide is a peptide amphiphile (PA). This polypeptide contains a hexadecyl lipid chain and a peptide sequence with two phenylalanine (FF) residues at the C-terminus. The FF residues drive self-assembly through hydrophobic and π-stacking interactions. It is designed based on the KLVFF core motif from the amyloid beta (Aβ) peptide. The serine protease α-chymotrypsin can cleave KKFFVLK at two sites, leading to products KKF with FVLK and KKFF with VLK. KKFFVLK forms nanotubes and helical ribbons at room temperature, which is associated with its substantial β-sheet secondary structure. This nanostructure causes light scattering and a partly cloudy appearance in solution. The ability of KKFFVLK to form specific nanostructures and its responsiveness to enzymatic cleavage make it an interesting candidate for applications such as enzyme-responsive delivery systems or in the study of self-assembly processes related to biomaterials and nanotechnology.
-
Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
-
References
- Dehsorkhi A, Hamley IW, Seitsonen J, Ruokolainen J. Tuning self-assembled nanostructures through enzymatic degradation of a peptide amphiphile. Langmuir. 2013 Jun 4;29(22):6665-72. doi: 10.1021/la401025r. Epub 2013 May 17. PMID: 23651310; PMCID: PMC3678293.
-
About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
-
Molar Concentration Calculator
-
Dilution Calculator
-
Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"