Human SCF/C-kit ligand (aa 1-189, His Tag) recombinant protein
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein short names
SL; CLO; FPH2; SCF; SHEP7; CONTRASTED; CON; MGF; SLF; SF; KL-1; STEEL; GB; DKFZP686F2250; C-KIT LIGAND; KITL; KITLG
KITLG; MGF; SCF
A DNA sequence encoding the amino acid (Met 1-Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a polyhistidine tag at the C-terminus.
The secreted recombinant human SCF (aa 1-189) consists of 175 amino acids and predicts a molecular mass of 19.9 kDa. The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.
> 92 % as determined by SDS-PAGE
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is 2-8 ng/mL.
Human SCF / C-kit ligand Protein (aa 1-189, His Tag) SDS-PAGE
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