Mouse SCF/C-kit ligand (His Tag) recombinant protein

Mouse SCF/C-kit ligand (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

Kit ligand

C-KIT LIGAND; GB; DKFZP686F2250; KITLG; KITL; SCF; FPH2; SHEP7; SL; CLO; KL-1; SF; SLF; STEEL; CONTRASTED; MGF; CON

P20826

Kitlg; Kitl; Mgf; Sl; Slf

Baculovirus-Insect Cells

A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 μg/ml (100 μl/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323.). The EC₅₀ for this effect is typically 0.5-5 ng/ml.
3. Measured by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells.
The ED₅₀ for this effect is 6-24 ng/mL.