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Product Name
KIFC1 antibody
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Description
KIFC1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive IF detected in Hela cells, HepG2 cells. Positive WB detected in rat testis tissue, HeLa cells, mouse testis tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 55-70 kDa
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HSET antibody; KIFC1 antibody; kinesin family member C1 antibody; Kinesin like protein 2 antibody; Kinesin like protein KIFC1 antibody; Kinesin related protein HSET antibody; KNSL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KIFC1 recombinant protein (Accession Number: BC000712). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using KIFC1 antibody Catalog No:112053 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
rat testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112053(KIFC1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112053(KIFC1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112053(KIFC1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)
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