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Product Name
KIFAP3 Polyclonal Antibody
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Description
Polyclonal antibody to KIFAP3
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
KIFAP3 antibody; FLA3 antibody; KAP-1 antibody; KAP-3 antibody; KAP3 antibody; SMAP antibody; Smg-GDS antibody; dJ190I16.1 antibody; kinesin-associated protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:100 -
Validations
Western blot - KIFAP3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KIFAP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunoprecipitation - KIFAP3 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug KIFAP3 antibody . Western blot was performed from the immunoprecipitate using KIFAP3 antibody at a dilition of 1:1000.
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Background
Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"