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Product Name
KIFAP3 antibody
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Description
KIFAP3 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 91 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ190I16.1 antibody; FLJ22818 antibody; KAP 3 antibody; KAP3 antibody; KIF3AP antibody; KIFAP3 antibody; kinesin assOCiated protein 3 antibody; SMAP antibody; Smg GDS antibody; Smg GDS assOCiated protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KIFAP3 recombinant protein (Accession Number: NM_014970). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112052(KIFAP3 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112052(KIFAP3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112052(KIFAP3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-KIFAP3 (IP:Catalog No:112052, 4ug; Detection:Catalog No:112052 1:500) with mouse testis tissue lysate 4000ug.
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Background
KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.
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References
- Gäken J, Mohamedali AM, Jiang J. A functional assay for microRNA target identification and validation. Nucleic acids research. 40(10):e75. 2012.
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