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Product Name
KIF22 antibody
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Description
KIF22 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KID antibody; KIF22 antibody; kinesin family member 22 antibody; Kinesin like protein 4 antibody; Kinesin like protein KIF22 antibody; KNSL4 antibody; OBP antibody; OBP 1 antibody; OBP 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KIF22 recombinant protein (Accession Number: NM_007317). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112007(KIF22 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:112007(KIF22 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:112007(KIF22 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-KIF22 (IP:Catalog No:112007, 4ug; Detection:Catalog No:112007 1:300) with HeLa cells lysate 4000ug.
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Background
KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).
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References
- Yan GR, Zou FY, Dang BL. Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study. Proteomics. 12(14):2391-9. 2012.
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