KEMPFPKYPVEP peptide

KEMPFPKYPVEP peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KEMPFPKYPVEP-OH

H-Lys-Glu-Met-Pro-Phe-Pro-Lys-Tyr-Pro-Val-Glu-Pro-OH

12

95.11%

C₇₀H₁₀₄N₁₄O₁₈S

1461.72

Synthetic

Powder

KEMPFPKYPVEP is a peptide identified from a screen of digested casein protein for the ability to improve spatial memory in amnesia mouse models. KEMPFPKYPVEP peptide was found to prevent scopolamine induced deficit in short term spatial memory, and also enhance long term object recognition memory in intact mice, with increased levels of dopamine and norepinephrine, but not serotonin being found in the frontal cortex. Previous epidemiological investigations have indicated that consuming dairy products can prevent cognitive decline, however only cheese fermented with fungi contains the KEMPFPKYPVEP peptide, which is found at the highest level in Camembert at 14.58-39.02 pmol per gram.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Ano et al (2019) Identification of a Novel Peptide from β-Casein That Enhances Spatial and Object Recognition Memory in Mice. J Agric Food Chem. 67(29) 8160 PMID: 31241932

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.