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Product Name
Kdm6b Polyclonal Antibody
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Description
Polyclonal antibody to Kdm6b
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
KDM6B antibody; JMJD3 antibody; lysine demethylase 6B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - Kdm6b Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using Kdm6b antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Western blot - Kdm6b Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using KDM6B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - Kdm6b Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Kdm6b Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using KDM6B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"