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  • Kdm6b Polyclonal Antibody
  • Kdm6b Polyclonal Antibody
  • Kdm6b Polyclonal Antibody
  • Kdm6b Polyclonal Antibody

Kdm6b Polyclonal Antibody

Cat.#: 161250

Special Price 174.2 USD

Availability: In Stock
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Product Information

  • Product Name
    Kdm6b Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to Kdm6b
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    KDM6B antibody; JMJD3 antibody; lysine demethylase 6B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - Kdm6b Polyclonal Antibody

    Western blot - Kdm6b Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using Kdm6b antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

    Western blot - Kdm6b Polyclonal Antibody

    Western blot - Kdm6b Polyclonal Antibody

    Western blot analysis of extracts of mouse brain, using KDM6B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunofluorescence - Kdm6b Polyclonal Antibody

    Immunofluorescence - Kdm6b Polyclonal Antibody

    Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Kdm6b Polyclonal Antibody

    Immunofluorescence - Kdm6b Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using KDM6B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"