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Product Name
KCNJ4 Polyclonal Antibody
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Description
Polyclonal antibody to KCNJ4
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
KCNJ4 antibody; HIR antibody; HIRK2 antibody; HRK1 antibody; IRK-3 antibody; IRK3 antibody; Kir2.3 antibody; inward rectifier potassium channel 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KCNJ4 (NP_004972.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - KCNJ4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KCNJ4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - KCNJ4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using KCNJ4 antibody at dilution of 1:100 (40x lens).
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Background
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"