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Product Name
KCNJ2 Polyclonal Antibody
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Description
Polyclonal antibody to KCNJ2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
KCNJ2 antibody; ATFB9 antibody; HHBIRK1 antibody; HHIRK1 antibody; IRK1 antibody; KIR2.1 antibody; LQT7 antibody; SQT3 antibody; inward rectifier potassium channel 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 318-427 of human KCNJ2 (NP_000882.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - KCNJ2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KCNJ2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"