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Product Name
KATNB1 antibody
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Description
KATNB1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HepG2 cells, mouse skeletal muscle tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 72-80 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KAT antibody; Katanin p80 subunit B1 antibody; KATNB1 antibody; p80 katanin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KATNB1 recombinant protein (Accession Number: NM_005886). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111916(KATNB1 antibody) at dilution of 1:300
IP Result of anti-KATNB1 (IP:Catalog No:111916, 3ug; Detection:Catalog No:111916 1:500) with HeLa cells lysate 3000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:111916(KATNB1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Katanin p80 WD40-containing subunit B1 is a protein that in humans is encoded by the KATNB1 gene. Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.
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References
- Jiang K, Hua S, Mohan R. Microtubule minus-end stabilization by polymerization-driven CAMSAP deposition. Developmental cell. 28(3):295-309. 2014.
- Hu WF, Pomp O, Ben-Omran T. Katanin p80 regulates human cortical development by limiting centriole and cilia number. Neuron. 84(6):1240-57. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"