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Product Name
KAT7 Polyclonal Antibody
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Description
Polyclonal antibody to KAT7
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
KAT7 antibody; HBO1 antibody; HBOA antibody; MYST2 antibody; ZC2HC7 antibody; lysine acetyltransferase 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-193 of human KAT7 (NP_008998.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - KAT7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KAT7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - KAT7 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using KAT7 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (By similarity). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1. Specifically represses AR-mediated transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"