Product Name

JNK antibody

Documents

Description

JNK Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human lung cancer tissue, mouse heart tissue. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, PC-3 cells, rat spleen tissue, RAW264.7 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 40-45 kDa, 50-55 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human, Mouse, Rat; other species not tested.

Alternative names

c Jun N terminal kinase 1 antibody; JNK antibody; JNK 46 antibody; JNK1 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; MAPK8 antibody; PRKM8 antibody; SAPK1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of JNK recombinant protein (Accession Number: NM_001323323). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111891(JNK antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111891(JNK antibody) at dilution of 1:50 (under 10x lens)

IP Result of anti-JNK (IP:Catalog No:111891, 3ug; Detection:Catalog No:111891 1:1000) with HeLa cells lysate 1000ug.

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:111891(JNK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ).

References

Lin CL, Zhang ZX, Xu YJ, Ni W, Chen SX. Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis. Chinese medical journal. 118(1):20-6. 2005.
Su CC, Chen JY, Din ZH. 13-acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression. Marine drugs. 12(10):5295-315. 2014.
Xu D, Su C, Song X. Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release. Chemical research in toxicology. 28(6):1326-37. 2015.

JNK antibody

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