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Product Name
JNK antibody
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Description
JNK Mouse Monoclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, C6 cells, Ethacrynic acid treated HeLa cells, HEK-293 cells, Jurkat cells. Observed molecular weight by Western-blot: 44 kDa, 55 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
c Jun N terminal kinase 1 antibody; JNK antibody; JNK 46 antibody; JNK1 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; MAPK8 antibody; PRKM8 antibody; SAPK1 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of JNK recombinant protein (Accession Number: NM_001323330). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107357(JNK Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:107357(JNK Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:107357(JNK Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of HeLa cells using Catalog No:107357(JNK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"