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Product Name
JMY antibody
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Description
JMY Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue, human brain tissue. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, HeLa cells. Observed molecular weight by Western-blot: 111 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
FLJ37870 antibody; JMY antibody; WHDC1L3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of JMY recombinant protein (Accession Number: BC130624). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111889(JMY Antibody) at dilution of 1:1000
IP Result of anti-JMY (IP:Catalog No:111889, 4ug; Detection:Catalog No:111889 1:600) with Jurkat cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:111889(JMY Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:111889(JMY Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:111889(JMY Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
Junction mediating and regulatory protein（JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"