-
Product Name
JMJD4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to JMJD4
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
JMJD4 antibody; jumonji domain containing 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human JMJD4 (NP_075383.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:1000 - 1:5000
-
Validations
Western blot - JMJD4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using JMJD4 antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"